Инструкция для OLYMPUS BX51WI

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INSTRUCTIONS

BX51WI

FIXED-STAGE UPRIGHT

This instruction manual is for the Olympus Fixed-Stage Upright Microscope Model BX51WI. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the
microscope. Retain this instruction manual in an easily accessible place near the work desk for
future reference.

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Оглавление инструкции
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    INSTRUCTIONS BX51WI FIXED-STAGE UPRIGHT MICROSCOPE This instruction manual is for the Olympus Fixed-Stage Upright Microscope Model BX51WI. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual
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    BX51WI CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 36 to 42) carefully. For the modules provided with instruction manuals, also read the
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    5 OTHER OBSERVATION METHODS 19-30 5-1 Differential Interference Contrast Observation ............................................................................... 19-23 1 Attaching the Analyzer 2 Attaching the Polarizer 3 Attaching the DIC Prisms (for Revolving Nosepiece) 4 Attaching the DIC
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    BX51WI IMPORTANT This microscope employs a UIS2 (UIS) (Universal Infinity System) optical design, and should be used only with UIS2 (UIS) eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series are also usable. For details, please consult Olympus or
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    4. In IR (infrared) observation, the U-CA or U-ECA magnification changer can be used only when the U-ETR3 or U-TR30IR observation is used. 5. In photomicrography with visible light, correct exposure may be impossible if the microscope is set for IR observation. Be sure to engage the provided IR cut
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    BX51WI MODULE NOMENCLATURE }The modules shown below are only the representative modules. As there are other modules which can be combined with the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer. Transmitted Arm BX-ARM or Reflected Light
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    CONTROLS }If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 36 to 42). Light path selector knob (Page 15) Interpupillary distance scale (Page 14) Reflected light mercury lamp housing (See the separately provided instruction manual.) Diopter adjustment ring (Page 14)
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    BX51WI Polarizer rotating dials (Page 23) Filter Turret Polarizer positions (Page 19)* Accepts the 32PO or 32POIR. Slider insertion/removal stopper (Page 10) Indication label attaching frame * Accepts the combination of a polarizer push ring and a 32 mm filter. The thickness should be no more than
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    Swinging Revolving Nosepiece WI-SRE3 Swing lever Confocality correction washers (Kinds of thickness: 10, 30 and 50 μm; three washers per kind) DIC mount hole (Page 20) Centering screw UIS2 (UIS) objective mount screws Centering knob Long-WD Universal Condenser WI-UCD Aperture iris diaphragm lever
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    BX51WI Differential Interference Contrast Prisms (For Condenser) }The WI-UCD condenser accepts two large and two small DIC prisms while the WI-DICD condenser accepts one large or small DIC prism. When selecting the brightfield (BF) light path using the WI-UCD, leave one DIC prism (large) mount
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    TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE }The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic observation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and IR DIC
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    BX51WI | ‰ Š … ƒ ‹Œ™ † ³ † š † @ ‡ ² ‡ Used only when the hand switch is not connected } Make a photocopy of the observation procedure pages and post it near your microscope. 9
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    USING THE CONTROLS 4-1 Microscope Base, Power Supply Unit (TH4) 1 ³ Controlling the Light Intensity (TH4) (Figs. 2 & 3) }See the separately provided instruction manual for details. 1. Make sure that the light intensity control knob @ is set to MIN (minimum voltage), then set the main switch ² to “
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    BX51WI 4-2 Focusing Block 1 Using the Pre-focusing Lever (Fig. 5) }The pre-focusing lever prevents collision between the specimen and objective and simplifies the focusing operation. After bringing the specimen into approximate focus with the coarse adjustment knob, turn the pre-focusing lever @ in
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    4-3 Stage (IX-SVL2) 1 Placing the Specimen (Fig. 8) 1. Place the specimen on the center of the stage. }The optional stage center plate (IX-CP50) makes it possible to observe a wide range of a big petri dish, etc. (Central hole diameter: 50 mm) Fig. 8 2 Moving the Specimen (Fig. 9) 1. The specimen
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    BX51WI 5 Using the Light Shield Sheet (Fig. 12) # The light shield sheet provided with the reflected light fluorescence illuminator is too small to be used with the BX51WI. Always use the light shield sheet provided with the BX51WI microscope frame. }During fluorescence observation using a
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    4-5 Observation Tube 1 Adjusting the Interpupillary Distance (Fig. 15) While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it
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    BX51WI 4 @ (Fig. 19) Eyepiece micrometer disks can be inserted into the WHN10X-H (or WHN10X) eyepieces. However, if the eyepiece does not have the helicoid adjustment facility and your eyesight is poor, you may have difficulties in focusing on the eyepiece micrometer disk. In this case, it is
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    4-6 Condenser 1 ² ³ | Fig. 21 Centering the Condenser (Figs. 21 & 22) 1. Set the aperture iris diaphragm lever @ to the open position. (Fig. 22) 2. Set the field iris diaphragm ring ² to the open position ( ¦). (Fig. 21) 3. Focus on the specimen using the 10X objective. 4. Close the field iris
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    BX51WI Aperture iris diaphragm image Aperture Iris Diaphragm 70-80% · The aperture iris diaphragm determines the numerical aperture of the illumination system. Matching the numerical aperture of the illumination system with that of the objective provides better image resolution and contrast, and
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    4-7 Immersion Objectives 1 @ ² Using Water Immersion Objectives }When the UMPlanFLN series, LUMPlanFLN series or XLUMPlanFl20XW objective is used, cultured tissue specimens which are often very thick can be observed by immersing the specimen, objective front lens and manipulator extremity in a
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    BX51WI OTHER OBSERVATION METHODS 5-1 Differential Interference Contrast Observation # The normal optical performance of DIC observation cannot be manifested if a plastic petri dish is used. }DIC prisms (for revolving nosepiece and condenser), an analyzer and a polarizer are required for DIC
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    ² ³ | @ Fig. 31 4 3 Attaching the DIC Prisms (for Revolving Nosepiece) (Fig. 31) }The DIC prisms for use in the revolving nosepiece include the WI-DICTHRA2 (high-resolution type) and WI-DICT2 (middle-contrast type). The revolving nosepieces in which a DIC prism can be inserted are the WI-SRE3 and
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    BX51WI With the WI-UCD Condenser (Figs. 32-34) ² }When selecting the brightfield (BF) light path using the WI-UCD, leave one DIC prism (large) mount position empty. 1. Remove the WI-UCD condenser from the microscope frame. 2. Remove the condenser cover @ by loosening the retaining screws ² using a
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    With the WI-DICD Condenser (Fig. 35) ³ ² @ }The WI-DICD should be attached after completing the polarizer position as described in item 5 . 1. Remove the WI-DICD condenser from the microscope frame. 2. Remove the two clamping screws @ using the Allen screwdriver provided with the microscope, then
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    4. Remove the eyepiece from the eyepiece sleeve, look into the sleeve, turn the polarizer rotation dial ² so that the black interference stripe (Fig. 37) is darkest, and tighten the clamping knob ³. 5. Engage an objective (as low-magnification as possible) in the light path, attach the condenser
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    5-2 Reflected Light Fluorescence Observation }Refer to the instruction manual of your reflected light fluorescence system. Illuminator Shutter WI-RSH }The shock during observation can be reduced by using this optional shutter in place of using the shutter built into the BX-URA2 or BX-RFA reflected
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    BX51WI 3. To enable IR observation, the following modules should be replaced with those based on the IR specifications. TV adapter IR TV camera Video contrast device such as the ARGUS10, 20 by Hamamatsu Photonics, monochrome video monitor (Note) Trinocular tune U-TR30IR U-ETR3 U-TR30-2 Analyzer
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    2 Attaching the IR Modules (Figs. 40 & 41) @ Thermal Reflection Filter 45SCF 1. Remove the collector lens of the U-LH100IR lamp housing @ by loosening the 3 clamping screws ² with an Allen wrench (width across flats of 2.5 mm). ² Fig. 40 | 2. While positioning the 45SCF filter ³ so that the arrow
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    BX51WI 3 DIC Observation Using IR Since IR light is harmful to your eyes, use the monitor observation whenever possible even in adjustments. 1. First, perform adjustments for DIC observation without using IR. }Do not mount the IR filter and see Section 5-1, “Differential Interference Contrast
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    5-4 Macro Reflected Light Fluorescence Observation }The macro reflected light fluorescence observation makes possible bright, low-magnification fluorescence observation by combining low-magnification fluorescence mirror units and fluorescence objective. 1 Introduction 1. For the low-magnification
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    BX51WI 2 Attaching the Modules (Figs. 42 & 43) Low-Magnification Fluorescence Mirror Units }Select the suitable mirror units for purpose of observation by referring to page 30. }If you want to fabricate optional mirror units, see page 30. · Mount the mirror units as indicated in the instruction
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    3 Filter Characteristics of Fluorescence Mirror Units Excitation Method Mirror Unit Dichroic Mirror Excitation Filter DM505 Barrier Filter BP460-490 U-MGFP/XL IB BA510IF U-MGFPA/XL BA510-550 U-MGFPA/XL Transmittance (%) Wavelength (nm) 4 For EGFP, S65T, RSGFP. (U-MGFPA/XL is for fluorochrome
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    BX51WI TROUBLESHOOTING GUIDE Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list,
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    Problem f ) Visibility of observed image is poor. · Image is not sharp. · Contrast is poor. · Details are poorly visible. Cause Remedy Page – Bubbles attached to the objective front Remove the bubbles. lens. – Too small quantity of solution in the petri Supply sufficient solution in the petri dish.
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    BX51WI Problem Cause Remedy Page 3. Coarse/Fine Adjustment Knobs a) Coarse adjustment knob is too The rotation tension adjustment ring is Fully loosen the ring by turning it counheavy to rotate. secured tightly. terclockwise. The pre-focusing lever is locked. Release the pre-focusing lever. b) The
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    SPECIFICATIONS Item Specification 1. Optical system UIS2 (UIS) (Universal Infinity System) optical system (Infinity correction) 2. Illumination system Transmitted Kohler illumination built in (FN 22) 12 V, 100 W long-life halogen bulb (pre-centered) Average life time: Approximately 2000 hr. when
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    BX51WI OPTICAL CHARACTERISTICS UIS series objectives not listed here can also be combined with this microscope. The following table shows the optical characteristics of combinations of eyepieces and objectives. The figure on the right shows the performance data engraved on the objectives. Objective
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    ASSEMBLY 9-1 Assembly Diagram The diagram below shows the sequence of assembly of the modules. The numbers indicate the order of assembly. The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult
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    BX51WI Revolving Arm, Revolving Nosepiece, Objectives Light shielding tube Light shielding tube (transmitted light arm adapter) Reflected light fluorescence illuminator BX-URA2 BX-RFA Transmitted light arm BX-ARM Light shielding tube The revolving nosepiece clamping screw is not used. Revolving arm
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    9-2 Detailed Assembly Procedures }When performing observation on a desktop, attach the provided rubber feet (x 4) to the front (x 2) and rear (x 2) parts on the bottom of the base. ³ 1 @ Attaching the Revolving Arm (Fig. 44) 1. Loosen the 2 clamping screws ² of the revolving arm @ using an Allen
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    BX51WI ³ | 3. Engage the objective on the back side in the light path. Adjust focusing, read the scale indication of the fine adjustment knob and obtain the difference from the value read in step 2 above. 4. The objective to use the washer is determined by the rotation direction of the fine
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    4 @ ³ | Fig. 49 5 ² ³ ³ @ Attaching the Waterproof Cover 40 (Fig. 50) }Attach the waterproof cover onto the condenser if required. The waterproof cover is applicable only to the WI-UCD, WI-DICD and WIOBCD condensers. 1. Fit the hole of the waterproof cover @ in the extremity of the condenser and
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    BX51WI 6 Attaching the Halogen Lamp Housing (Figs. 51-54) Attaching the Halogen Bulb @ ² Fig. 51 ³ Fig. 52 ƒ | … }The applicable lamp bulb model is the 12V100WHAL-L (PHILIPS 7724) or the 12V50WHAL-L (LIFE JC). 1. Fully loosen the clamping screw @ at the top of the lamp housing using the Allen
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    † @ Fig. 55 ² … ƒ 7 Attaching the Cross Stage (Figs. 55 & 56) }When using a commercially marketed bridge stage, attach it by referring to its instruction manual. 1. Align the two WI-FSH fixed stage adapters @ with the front of the microscope base and clamp the adapters by tightening the hex-socket
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    BX51WI PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus
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    Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Approval Organization Printed or Embossed Harmoniza- Alternative Marking Utilizing tion Marking (May be located on Black-Red-Yellow Thread (Length jacket or insulation of internal wir- of color section in mm)
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    10 BX51WI LAMP HOUSING INSPECTION SHEET {Study the instruction manual for the lamp housing before inspection. {For safe use of the lamp housing, we recommend performing the following inspection periodically (every time you replace the lamp bulb and at least every 6 months). {The table below
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    MEMO
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    Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan EC REP Wendenstraße 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. One Corporate Drive, Orangeburg, NY 10962, U.S.A. 491B River Valley Road, #12-01/04 Valley Point
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