Инструкция для OLYMPUS BX61WI

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This instruction manual is for the Olympus Fixed-Stage Motorized Upright Microscope Model BX61WI.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this
microscope, we recommend that you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.

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Оглавление инструкции
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    INSTRUCTIONS BX61WI FIXED-STAGE MOTORIZED UPRIGHT MICROSCOPE This instruction manual is for the Olympus Fixed-Stage Motorized Upright Microscope Model BX61WI. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you
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    This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive. NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC
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    BX61WI CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 42 to 49) carefully. For the modules provided with instruction manuals, also read the
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    5 OTHER OBSERVATION METHODS 24-35 5-1 Differential Interference Contrast Observation .............................................................................. 24-28 1 Attaching the Analyzer 3 Attaching the DIC Prisms (for Revolving Nosepiece) 2 Attaching the Polarizer 4 Attaching the DIC
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    BX61WI IMPORTANT This microscope employs a UIS2 (UIS) (Universal Infinity System) optical design, and should be used only with modules designed for the BX2 series (which belong to the Olympus BX series). For the applicable modules, please consult Olympus or the latest catalogues. Less than optimum
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    2 1 Fig. 1 5. When moving the microscope, disconnect the reflected light illuminator, observation tube and transmitted light lamp housing and carefully carry the microscope by the base (front edge) 1 and the grasping part on the rear of the arm 2 as shown in Fig. 1. (Weight: approx. 15 kg.) Also be
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    BX61WI 1 Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. The U-SWTR-3 super-widefield observation tube (FN 26.5) cannot be used with the BX61WI microscope. 3. The BX61WI microscope can be used with an intermediate
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    MODULE NOMENCLATURE }The modules shown below are only the representative modules. As there are other modules which can be combined with the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer. CAUTION The Z board combined with the BX61WI must always
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    BX61WI CONTROLS }If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 42 to 49). Reflected light fluorescence illuminator (See the separately provided instruction manual.) Light path selector knob (Page 20) Reflected light mercury lamp housing (See the separately provided
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    Left Side View of Microscope Frame Focus adjustment knob (Page 15) Objective escape/return button (Page 16) F/C button (Page 16) F: Fine adjustment C: Coarse adjustment Frost filterinsertion/removal lever (Page 16) Light Preset switch (Page 14) Lamp ON-OFF switch Filter Turret Polarizer rotating
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    BX61WI Revolving Arm WI-NPA # Note that the revolving arm can be mounted only before the reflected light illuminator or transmitted light arm and the IX-SVL2 stage are mounted (page 45). }This revolving arm accepts the U-SLRE, WI-SNPXLU2 or WI-SRE3 revolving nosepiece. Grounding screw hole (M3)
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    Long-WD Universal Condenser WI-UCD Aperture iris diaphragm lever (Page 22) Quarter-wave plate clamping knob Turret (4 positions) Optical element index mount (Page 26) Quarter-wave plate rotation ring (Page 28) DIC prism replacement cover Long-WD DIC Condenser WI-DICD DIC prism (Large) mount
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    BX61WI Long-WD Oblique Condenser WI-OBCD Aperture iris diaphragm lever (Page 22) Oblique iris insertion/removal knob (Page 22) High-Resolution DIC Prism A WI-DICTHRA2 DIC Prism WI-DICT2 }This prism can be mounted in the DIC prism position of the WI-SNPXLU2 or WI-SRE3. Applicable condensers
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    }The following modules are required to eliminate electrical noise, which may affect the potential measurement data during patch clamping. Halogen Lamp Power Supply TH4 (See the separately provided instruction manual for details.) Hand Switch TH4-HS Focus Adjustment Knob Interface U-IFFH Microscope
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    BX61WI Focus Adjustment Knob Unit U-FH The button functions described below are those when the microscope is used stand-alone. The buttons functions inside are the initial setups for PC control (remote operation). The button functions can also be assigned as required by the user. For the
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    TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE }The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic observation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and IR DIC
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    BX61WI (Note) Controls with numbers inside can perform the same functions as the controls with numbers inside ¦. However, the function of control 9 is not available when the U-FH is in use. 6 b 3 a 8 9 7 cdf 9 5 e 4 9 3 9 1 Control Box BX-UCB * The U-ZPCB (T2) Z board can be mounted here. See page
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    USING THE CONTROLS 4-1 Microscope Frame 1 3 1 4 (Fig. 3) 1. Press the light intensity control button 1 to increase the voltage and make illumination brighter. Pressing the button 2 makes the illumination darker. 2. The numerals to the left of the lamp voltage indicator LEDs 3 indicate the reference
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    BX61WI 4 1 2 (Fig. 5) }Filters with a diameter of 32 mm can be inserted in positions 1 to 4. 1. Positions 1 and 2 are rotatable. When the 32PO or 32POIR polarizer is placed in either position, the polarizer can be fixed by means of the push ring (made of white plastic). }When filter position 1 is
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    2 F/C Button (Fig. 7) }The F/C button switches the function of the focus adjustment knob 1 and focus adjustment dial between F (fine) and C (coarse). (For safety, the F/C button is set automatically to F at the moment the main switch of the BX-UCB control box is set to “ I ” (ON).) · Each press of
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    BX61WI 4-3 Stage (IX-SVL2) 1 Placing the Specimen (Fig. 11) 1. Place the specimen on the center of the stage. }The optional stage center plate (IX-CP50) makes it possible to observe a wide range of a big petri dish, etc. (Central hole diameter: 50 mm) Fig. 11 2 Moving the Specimen (Fig. 12) 1. The
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    5 Using the Light Shielding Sheet (Fig. 15) # The light shielding sheet provided with the reflected light fluorescence illuminator is too small to be used with the BX61WI. Always use the light shielding sheet provided with the BX61WI. }During fluorescence observation using a low-magnification
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    BX61WI 4-5 Observation Tube 1 Adjusting the Interpupillary Distance (Fig. 18) While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so
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    4 1 Using Eyepiece Micrometer Disks (Fig. 22) Eyepiece micrometer disks can be inserted into the WHN10X-H (or WHN10X) eyepieces. Use 24 mm dia. x 1.5 mm micrometer disks. Following Fig. 22, remove the micrometer mounting frame 2 from the eyepiece and place a micrometer disk 1 into the mounting
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    BX61WI 4-6 Condenser 1 4 2 3 Fig. 24 Centering the Condenser (Figs. 24 to 26) 1. Set the aperture iris diaphragm lever 1 to the open position. (Fig. 25) 2. Set the field iris diaphragm ring 2 to the open position ( ¦). (Fig. 24) 3. Focus on the specimen using the 10X objective. 4. Close the field
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    Aperture Iris Diaphragm Aperture iris diaphragm image 70-80% · The aperture iris diaphragm determines the numerical aperture of the illumination system. Matching the numerical aperture of the illumination system with that of the objective provides better image resolution and contrast, and also
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    BX61WI 4-7 Water Immersion Objectives 1 1 2 Using Water Immersion Objectives }When the UMPlanFLN series, LUMPlanFLN series or XLUMPlanFl20XW objective is used, cultured tissue specimens which are often very thick can be observed by immersing the specimen, objective front lens and manipulator
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    OTHER OBSERVATION METHODS 5-1 Differential Interference Contrast Observation # The normal optical performance of DIC observation cannot be manifested if a plastic petri dish is used. }DIC prisms (for revolving nosepiece and condenser), an analyzer and a polarizer are required for DIC observation.
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    BX61WI 3 2 3 4 1 Fig. 34 4 Attaching the DIC Prisms (for Revolving Nosepiece) (Fig. 34) }The DIC prisms for use in the revolving nosepiece include the WDICTHRA2 (high-resolution type) and WI-DICT2 (middle-contrast type). The revolving nosepieces in which a DIC prism can be inserted are the WISRE3
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    With the WI-UCD Condenser (Figs. 35 - 37) 2 }When selecting the brightfield (BF) light path using the WI-UCD, leave one DIC prism (large) mount position empty. 1. Remove the WI-UCD condenser from the microscope frame. 2. Remove the condenser cover 1 by loosening the retaining screws 2 using a coin,
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    BX61WI With the WI-DICD Condenser (Fig. 38) 3 2 }The WI-DICD should be attached after completing the polarizer position as described in item 5 . 1. Remove the WI-DICD condenser from the microscope frame. 2. Remove the two clamping screws 1 using the Allen screwdriver provided with the microscope,
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    4. Remove the eyepiece from the eyepiece sleeve, look into the sleeve, turn the polarizer rotation dial 2 so that the black interference stripe (Fig. 40) is darkest, and tighten the clamping knob 3. 5. Engage an objective (as low-magnification as possible) in the light path, attach the condenser
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    BX61WI 5-2 Reflected Light Fluorescence Observation }Refer to the instruction manual of your reflected light fluorescence system. If you are using a reflected light illuminator which is not motorized, it is recommended to use the WI-RSH illuminator shutter. Illuminator Shutter WI-RSH }The shock
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    3. To enable IR observation, the following modules should be replaced with those based on the IR specifications. TV adapter IR TV camera Video contrast device such as the ARGUS10.20 by Hamamatsu Photonics, monochrome video monitor (Note) Trinocular tune U-TR30IR U-ETR3 U-TR30-2 Analyzer WI-ANIR or
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    BX61WI 2 1 Attaching the IR Modules (Figs. 43 & 44) Thermal Reflection Filter 45SCF 1. Remove the collector lens of the U-LH10IR lamp housing 1 by loosening the 3 clamping screws 2 with an Allen wrench (width across flats of 2.5 mm). 2 Fig. 43 4 2. While positioning the 45SCF filter 3 so that the
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    3 DIC Observation Using IR Since IR light is harmful to your eyes, use the monitor observation whenever possible even in adjustments. 1. First, perform adjustments for DIC observation without using IR. }Do not mount the IR filter and see Section 5-1, “Differential Interference Contrast Observation”
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    BX61WI 5-4 Macro Reflected Light Fluorescence Observation }The macro reflected light fluorescence observation makes possible bright, low-magnification fluorescence observation by combining low-magnification fluorescence mirror units and fluorescence objective. 1 Introduction 1. For the
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    2 Attaching the Modules (Figs. 45 & 46) Low-Magnification Fluorescence Mirror Units }Select the suitable mirror units for purpose of observation by referring to page 35. }If you want to fabricate optional mirror units, see page 35. · Mount the mirror units as indicated in the instruction manual of
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    BX61WI 3 Filter Characteristics of Fluorescence Mirror Units Excitation Method Mirror Unit Dichroic Mirror Excitation Filter DM505 Barrier Filter BP460-490 U-MGFP/XL IB BA510IF U-MGFPA/XL BA510-550 U-MGFPA/XL Transmittance (%) Wavelength (nm) 4 For EGFP, S65T, RSGFP. (U-MGFPA/XL is for fluorochrome
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    TROUBLESHOOTING GUIDE Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please
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    BX61WI Problem Cause Remedy Page The objective in use is not designed for Replace with a specified objective for UIS2 (UIS) series. UIS2 (UIS) series. 41 The condenser is set to too low a posi- Adjust the condenser height. tion. 21 The aperture iris diaphragm is closed too Open it sufficiently.
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    Problem Cause Remedy Page 2. Electrical System a) The bulb intermittently lights and The bulb is nearly burnt out.. Replace the bulb. goes out. A cord or connector is not properly con- Connect cords and plugs securely. nected. b) The lamp bulb burns out soon after The bulb in use is not the
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    BX61WI SPECIFICATIONS Item Specification 1. Optical system UIS2 (UIS) (Universal Infinity System) optical system (Infinity correction) 2. Illumination system Transmitted Kohler illumination built in (FN 22) 12 V, 100 W long-life halogen bulb (pre-centered) 12V100WHAL-L (PHILIPS 7724) or 12 V, 50 W
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    Item 8. Operating environment 40 Specification · · · · Indoor use Altitude: Max. 2000 m Ambient temperature: 10° to 40°C (50°F to 104°F) Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at
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    BX61WI OPTICAL CHARACTERISTICS UIS series objectives not listed here can also be combined with this microscope. The following table shows the optical characteristics of combinations of eyepieces and objectives. The figure on the right shows the performance data engraved on the objectives. Objective
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    ASSEMBLY 9-1 Assembly Diagram The diagram below shows the sequence of assembly of the modules. The numbers indicate the order of assembly. The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult
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    BX61WI Revolving Arm, Revolving Nosepiece, Objectives Light shielding tube Turret Reflected light fluorescence illuminator BX-RFAA BX-URA2 BX-RFA Light shielding tube (Transmitted light arm adapter) Transmitted light arm BX-ARM Light shielding tube The revolving nosepiece clamping screw is not
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    9-2 Detailed Assembly Procedures # Be always sure to use the U-ZPCB(T2) Z board which is designed to be compatible with the BX61WI. The following phenomena will occur with the U-ZPCB even though the correct setup is performed. · The rotation direction of the focus adjustment knob is opposite to the
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    BX61WI }When performing observation on a desktop, attach the provided rubber feet (x 4) to the front (x 2) and rear (x 2) parts on the bottom of the base. Note that the rubber feet are not necessary when installing the microscope on an anti-vibration bench. 3 1 1 Attaching the Revolving Arm (Fig.
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    3. Engage the objective on the back side in the light path. Adjust focusing, read the scale indication of the fine adjustment knob and obtain the difference from the value read in step 2 above. 4. The objective to use the washer is determined by the rotation direction of the fine adjustment dial 2.
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    BX61WI 3 4 4 1 Fig. 54 5 2 3 3 Attaching the Waterproof Cover (Fig. 55) }Attach the waterproof cover onto the condenser if required. The waterproof cover is applicable only to the WI-UCD, WI-DICD and WI-OBCD condensers. 1. Fit the hole of the waterproof cover 1 in the extremity of the condenser and
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    6 Attaching the Halogen Lamp Housing (Figs. 56 to 59) Attaching the Halogen Bulb 1 2 Fig. 56 3 Fig. 57 5 6 }The applicable lamp bulb model is the 12V100WHAL-L (PHILIPS 7724) or the 12V50WHAL-L (LIFE JC). 1. Fully loosen the clamping screw 1 at the top of the lamp housing using the Allen screwdriver
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    BX61WI 7 1 Fig. 60 6 2 5 7 Attaching the Cross Stage (Figs. 60 & 61) }When using a WI-XYS bridge stage, attach it by referring to its instruction manual. 1. Align the two WI-FSH fixed stage adapters 1 with the front of the microscope frameand clamp the adapters by tightening the hex-socket screws
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    10 LAMP HOUSING INSPECTION SHEET {Study the instruction manual for the lamp housing before inspection. {For safe use of the lamp housing, we recommend performing the following inspection periodically (every time you replace the lamp bulb and at least every 6 months). {The table below identifies the
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    Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan EC REP Wendenstraße 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. One Corporate Drive, Orangeburg, NY 10962, U.S.A. 491B River Valley Road, #12-01/04 Valley Point
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