Инструкция для OLYMPUS LV200

(скачивание инструкции бесплатно)
Формат файла: PDF
Доступность: Бесплатно как и все руководства на сайте. Без регистрации и SMS.
Дополнительно: Чтение инструкции онлайн
background image

LV200 Installation: 
 

1)

 

Place the system in a dark room if possible or avoid direct strong light! 

2)

 

Install system table.  

3)

 

Place LV200 onto table right above the prepared whole. 

4)

 

Don’t place the PC, controllers onto the table (vibrations) 

5)

 

Connect transmitted light source  

6)

 

If you want to use filter wheels in cellM connect UCB with PC 

7)

 

The filter wheels are marked at the cable with FW1 and FW2. Connect them in the vice 
versa 
order into UCB ( FW1 in 2. and FW2 in 1.)  ! 
Choose “IX/51/71” in OBS config 
Choose “Contrast inserts” for upper filter wheel including blockers 
Choose “Filter Cubes” for lower filter wheel (empty in standard configuration) 

8)

 

Connect cables for temperature control (connection cable at the back of LV200) 

9)

 

Screw in the tube lens carefully from the bottom 

10)

 

Camera installation: 

a)

 

Fix the c-mount adaptor onto the camera 

b)

 

Remove the small black metal part at the front to get access to the mounting 
screw. 

c)

 

Use the long hex wrenge 3 (small whole at the front) to fix the camera. 

Caution 

the camera may fall down !!

 

11)

 

The camera is used without a trigger cable to avoid heating of the chip 

12)

 

To change the objectives you have to loose the screws for the incubation chamber. 
During a 
demo please leave this screws loose, to have the possibility of changing the objective 

13)

 

You can only use 35 mm Petri dishes (and with some limitations also slides). Best glass 
bottom Petri dishes 

 

 
 
 
Important points for Software:  only for cellM  (until now not possible with Andor 
iKonM) 
 

1)

 

The integration times will be much higher as in fluorescence may be 100 000 ms or 
even 1 000 000 ms. 

2)

 

The 100 s limit of integration time is solved from version 3.1 

3)

 

In the standard configuration there are light blockers in every second position of the 
filter wheel. In bioluminescence mode use them to prevent light passing the light 
guide. Turn off light source during long experiments. Lowest energy level and almost 
closed shutter at the light source gives 
enough light. 

4)

 

Bright field. Change light blockers by hand switch or PC after you have decreased the 
integration time lower than 50 ms.  

5) Acquisition using experiment manager: 

 
don’t use trigger cable. The chip will get too warm. 

 

only use “simple acquisition” instead of image type button 

 

use “non synchronous” mode  

 

ad a time loop around the acquisition (is needed for nonsynchronous mode) 

1
2
Оглавление инструкции
  • Страница 1 из 3
    LV200 Installation: 1) 2) 3) 4) 5) 6) 7) Place the system in a dark room if possible or avoid direct strong light! Install system table. Place LV200 onto table right above the prepared whole. Don’t place the PC, controllers onto the table (vibrations) Connect transmitted light source If you want to
  • Страница 2 из 3
    change the gain in live mode to the needed value. Experiments: 1) The customer has to know the difference between fluorescence and luminescence. Signals are much weaker (factor 1000 to 1 000 000 !!!) 2) Highest intensity with low magnifications! 3) Due to 0.2 tube lens magnifications of a 20x lens
  • Страница 3 из 3