Инструкция для OLYMPUS SCAN R HARDWARE

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INSTRUCTIONS 

 

AUTOMATED IMAGE ACQUISITION 

HARDWARE 

This instruction manual describes the hardware components controlled by the  
Olympus 

 Automated Image Acquisition Software. To ensure safety, obtain 

optimum performance and familiarize yourself fully with the use of these products, we 
recommend that you study this manual thoroughly before operation. Together with 
this manual, please also read the 

 Automated Image Acquisition Software 

manual as well as the manuals of all other devices controlled by this software in order 
to understand general operation methods. Retain this manual in an easily accessible 
place near a system for future reference.

 

®

 

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Оглавление инструкции
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    ® INSTRUCTIONS AUTOMATED IMAGE ACQUISITION HARDWARE This instruction manual describes the hardware components controlled by the Automated Image Acquisition Software. To ensure safety, obtain Olympus optimum performance and familiarize yourself fully with the use of these products, we recommend that
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    OLYMPUS SOFT IMAGING SOLUTIONS GMBH Rupert-Mayer-Strasse 44 D-81379 München Tel: +49 89 - 89 55 805 660 Fax +49 89 - 89 55 805 6606 Email: info@olympus-sis.com www.olympus-sis.com
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    Imaging Excellence We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever
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    Declaration of Conformity Robert-Koch-Strasse 9 D-82152 Planegg declares under its own responsibility that the following products Imaging Station for Life Science Applications * to which this declaration refers are in conformity with the Low Voltage Directive 73/23/EEC and the EMC Directives
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    Hardware Manual Contents Contents 1 Introduction and Overview ................................................................................1 1.1 Abstract ........................................................................................................2 1.2 Technical
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    Contents
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    Hardware Manual Chapter 1 – Introduction and Overview 1 Introduction and Overview Thank you very much for purchasing Olympus' state of art Screening Station for Life Science and for your confidence in our products and services. It is Olympus main objective to provide you with solutions able to meet
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    2 Chapter 1 – Introduction and Overview Analysis Software, the Acquisition Software as well as the hardware The components of the Olympus Screening Station for Life Sciences are for research use only. 1.1 Abstract The Screening Station for Life Science scan^R is based on the hardware of the cell^R
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    Hardware Manual Chapter 1 – Introduction and Overview 1.2 Technical Support If you find any information missing in this manual or you need additional support, please contact your local Olympus representative. 1.3 Symbols The following symbols are placed on the Illumination System MT20. Study the
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    4 Chapter 1 – Introduction and Overview 1.4 Checklist: Standard scan^R Components Before assembly, please check whether you have the complete set of standard scan^R system components as listed below. a Microscope IX81 with control unit IX2-UCB b Illumination System MT20 consisting of: — MT20
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    Hardware Manual Chapter 2 – Illumination System MT20 2 Illumination System MT20 This chapter refers only to the fluorescence Illumination System MT20. Before using this unit together with the other system components, the microscope and associated devices, make sure that you have carefully read and
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    6 Chapter 2 – Illumination System MT20 2.1 Safety Precautions and Handling Instructions For Laboratory Use Only. Danger: Ultraviolet radiation is emitted from this product. Avoid exposure. ALWAYS WARE PROTECTIVE CLOTHING. EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER. ALWAYS WARE
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    Hardware Manual f Chapter 2 – Illumination System MT20 The Illumination System MT20 must be placed on an even, stable and flat surface and standing on its supports. Make sure that no cloth or paper can clog the air inlet slits at the bottom of the housing. g The arc burner has to be exclusively one
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    8 Chapter 2 – Illumination System MT20 2.1.2 Special Safety Precautions Regarding the Arc Burners a Handling the arc burner bulb is potentially dangerous. Wear a protective mask, leather gloves and a thick long-sleeved shirt or coat during handling and disposal (see below). b The arc burner bulb is
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    Hardware Manual Chapter 2 – Illumination System MT20 2.1.3 Handling Instructions and Recommendations a The Illumination System MT20 is a precision instrument. Handle it with great care and avoid subjecting it to sudden or severe impact. Also connect each cable gently. b The built-in ventilating fan
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    10 Chapter 2 – Illumination System MT20 2.1.4 Safety Labels The following symbols are found on the Illumination System MT20. Study the meaning of the symbols and always use the equipment in the safest possible manner. Lamp housing safety label Label Note Location Carefully read the instructions on
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    Hardware Manual Chapter 2 – Illumination System MT20 2.2 Illumination System MT20 Overview 2.2.1 The Front Panel FIBER lock Fiber port LOCK BURNER ADJUSTMENT Light sensor connector LED: BURNER ON LED: Status Filter wheel connector CONTROLLER connector LOCK: 2 mm hex-wrench opening to lock or
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    12 Chapter 2 – Illumination System MT20 2.2.2 Top: the Burner Flap Opening for a 3 mm hex-wrench Access to the arc burner for replacement Before opening: Wait until system has cooled down, then switch main power off! 2.2.3 Right Side: the Filter Wheel Flap The flap has to be closed and the
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    Hardware Manual Chapter 2 – Illumination System MT20 2.2.4 The Back Panel Arc burner cooling air outlet Main power switch, fuse-holder and AC power input connector. Do not block ventilation slits! Keep 10 cm distance from wall. 2.2.5 Operation Only operate the system when completely installed and
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    14 Chapter 2 – Illumination System MT20 2.2.6 Specifications and Technical Data of the MT20 Dimensions and Weight: H 354 x W 236 x D 315 mm, 13 kg Power supply rating: 100 to 120 V and 200 to 240 V AC, 50 – 60 Hz (47 – 63 Hz), 3.9 / 1.6A Fuse: 250VAC, T4AH (5 x 20mm) Enclosure rating: IP20 Short
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    Hardware Manual Chapter 2 – Illumination System MT20 — FILTER WHEEL: sub-D, 15-pin connection — LIGHT SENSOR: 3 pin connection to light sensor (epi-fluorescence illuminator) Connectors, MT20 rear: — 3-pin AC input plug with ground — Main switch (I: on, O: off) Optical fiber: minimum bend diameter
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    16 Chapter 2 – Illumination System MT20
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    Hardware Manual Chapter 3 – Epi-Fluorescence Illuminator 3 Epi-Fluorescence Illuminator This chapter explains the details of the illuminator that couples the illumination system MT20 to the IX81 microscope. 3.1 3.1.1 3.1.2 3.1.3 3.1.4 3.1.5 Features
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    18 Chapter 3 – Epi-Fluorescence Illuminator 3.1 Features The MT20 epi-fluorescence illuminator is a tube which houses the different lenses and features the fiber port, the filter slider, the adjustments screws to center the field of illumination and the light sensor to measure the light output
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    Hardware Manual Chapter 3 – Epi-Fluorescence Illuminator 3.1.2 Adjustment Screws for Centering the Field of Illumination The illumination has to be centered in order to illuminate the entire chip of the CCD camera. No tool is needed to turn the screws. See Chapter 7.4, Centering the Illumination.
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    20 Chapter 3 – Epi-Fluorescence Illuminator
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 4 System Assembly and Adjustment The following chapters explain how all the different hardware modules of the scan^R Screening Station have to be connected for correct operation. 4.1 4.2 4.2.1 4.2.2 4.2.3 4.2.4 4.2.5 4.3 4.3.1 4.3.2 4.3.3
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    22 Chapter 4 – System Assembly and Adjustment It is the user’s responsibility to provide a sufficient and stable electrical power supply network for the scan^R system. Olympus rejects any warranty claims for any power break down follow up damage caused by an insufficient power supply network. 4.1
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 4.2 Connecting the Modules of the scan^R Screening Station 4.2.1 Connecting the MT20 1. Connect the power cord to the main power connector at the MT20 rear panel and to a power outlet. Caution: Make sure that the MT20's main power switch is
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    24 Chapter 4 – System Assembly and Adjustment 6. For the connection of the other connector of the fiber see the following chapter. UV radiation output; do NOT disconnect fiber while burner is switched ON. 4.2.2 Mounting and Connecting the Epi-Fluorescence Illuminator See also the photo in Chapter
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 4.2.3 Connecting the Camera The camera has to be mounted on the microscope camera port using a c-mount adapter as described in the microscope manual. 4.2.3.1 Camera Hamamatsu Orca AG 1. Make sure that the camera controller unit is switched
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    26 Chapter 4 – System Assembly and Adjustment USB port for cell^R hardware key 2 BNC port for camera trigger cable Controller serial port for motorized stage connection Binder plug for ODB device power supply Lemo plug to connect with ODB device (U_FFWO or IX2_FRFACA) SMB port for PIFOC controller
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    Hardware Manual 3. Go to Properties Chapter 4 – System Assembly and Adjustment General. 4. Make sure that Internet Protocol (TCP/IP) is activated and go to Properties. 5. Use the IP address 42.42.42.17 and the Subnet mask 255.255.255.0. 6. Confirm with OK. And go to the Advanced tab. 7. Make sure
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    28 Chapter 4 – System Assembly and Adjustment burner socket reflector anode cable tip-off nut heat sink 5. Tighten the lock screw gently but firmly until the screw is blocked; force is not necessary. The lock screw is spring loaded; you will feel the resistance of the spring once the screw is
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 2. Check if the burner type listed is correct. If not, click the Exchange burner button to open the Select burner type… dialog box that enables you to choose the type of arc burner. Confirm by clicking Ok. If you install a different type of
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    30 Chapter 4 – System Assembly and Adjustment 5. Open the shutter by clicking on the Shutter OPEN/CLOSE button. 6. The light output is indicated by the two blue bars. They do not represent an absolute value but are rescaled frequently. The maximum that was reached so far is indicated by the red
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 4.3.3 Filter Exchange 1. Start the OBS System Configuration software as described in the chapter above and go to the Excitation Filter page. Select the filter position that you want to exchange with the slider bar. 2. Open the flap at the
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    32 Chapter 4 – System Assembly and Adjustment 3. Close the flap. The system will perform a mechanical initialization that takes about 18 seconds. If you listen closely you can hear the filter wheel and attenuator disk turn. After initialization the STATUS LED is turned on automatically to indicate
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 4.4 Centering the Illumination 1. Put a fluorescent sample onto the microscope stage. Most suitably the fluorescence covers the entire field-of-view. 2. Start the scan^R acquisition software and go to Edit Scan Acquisition. 3. Move a
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    34 Chapter 4 – System Assembly and Adjustment 4.5 Optimizing the Illuminator Tube Position For an optimally homogeneous illumination of the specimen, it is necessary for the light cone leaving the illuminator exit lens to match the position of the objective back aperture. Radial intensity gradients
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    Hardware Manual Chapter 4 – System Assembly and Adjustment Standard and L-shape illuminators; the arrows mark the distances that have to be adjusted. Optimal distance between flange and illuminator tube end [mm] Objectives Standard L-shape A 231 41 B 221 31 C 211 21 The illumination will still be
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    36 Chapter 4 – System Assembly and Adjustment 4.6 Connecting the Controller of the Motorized Stage Back panel of the Corvus stage controller In this description it is assumed that the motorized stage is mounted on the microscope frame as designed by the manufacturer. 1. Make sure that the main
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 4.7 PC-to-Controller Network Connection Communication between the MSWindows imaging PC and the scan^R Real-time Controller is realized through an ordinary, albeit internal, network connection via a network card. The MSWindows status bar
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    38 Chapter 4 – System Assembly and Adjustment 13. Return to the CTR Properties window with OK. 14. Click on the Configure button and go to the Advanced tab of the Realtek window that opens. 15. Select the Property Link Speed/Duplex Mode and then the Value 10Mbps/Full Duplex from the shortlist.
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    Hardware Manual Chapter 4 – System Assembly and Adjustment 39
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    ® OLYMPUS LIFE SCIENCE EUROPA GMBH Postfach 10 49 08, 20034, Hamburg, Germany Printed in Germany 2008 12 M 2.1.0.14
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