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Оглавление инструкции
Страница 1 из 38 INSTRUCTIONS CKX41/CKX31 CULTURE MICROSCOPES This instruction manual is for the Olympus Culture Microscopes Models CKX41 and CKX31. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly
Страница 3 из 38 CKX41/CKX31 CONTENTS IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3 1 NOMENCLATURE 4-5 2 CONTROLS 6-7 3 SUMMARY OF OBSERVATION PROCEDURE 4 USING THE CONTROLS 8 9-15 4-1 Microscope Frame
Страница 5 из 38 CKX41/CKX31 IMPORTANT The difference between the CKX31 and CKX41 microscopes lie in the following basis systems. CKX31 CKX41 Observation tube Binocular tube fixed Replaceable* Stage center plate – Replaceable** Not mountable Mountable Reflected fluorescence system * The
Страница 6 из 38 9. The microscope system is unstable when the large camera back is attached. When pulling out the film, be sure to hold the microscope with one hand. 10. Always turn the light intensity control knob 5 gently. Do not attempt to turn it beyond the stop position. (Fig. 2) Safety Symbols The following
Страница 7 из 38 CKX41/CKX31 2 Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be
Страница 10 из 38 CONTROLS CKX31 }If you have not yet assembled the microscope, read chapter 9, “ASSEMBLY” (pages 25 to 29). Filter holder (Page 29) Phase contrast slider (Page 16) Aperture iris diaphragm lever (Page 15) Interpupillary distance scale (Page 11) Slider centering knob storage holes Diopter adjustment
Страница 11 из 38 CKX41/CKX31 CKX41 }If you have not yet assembled the microscope, read chapter 9, “ASSEMBLY” (pages 25 to 29). Filter holder (Page 29) Interpupillary distance scale (Page 11) Phase contrast slider (Page 16) Aperture iris diaphragm lever (Page 15) Diopter adjustment ring (Page 12) Standard stage
Страница 12 из 38 SUMMARY OF OBSERVATION PROCEDURE 1. Set the main switch 1 to “ I ” (ON) and turn the light intensity control knob 2 to obtain appropriate brightness. (Page 9) ² @ 2. When using the U-TR30-2 trinocular tube, push in the light path selector knob 3 to set the light path at 100% for binocular
Страница 13 из 38 CKX41/CKX31 USING THE CONTROLS 4-1 Microscope Frame 1 Turning On the Light Source (Fig. 3) Set the main switch 1 on the side panel of the microscope frame to “ I ” (ON). @ Fig. 3 2 Adjusting the Brightness (Fig. 4) Turn the light intensity control 1 clockwise to raise the voltage and increase the
Страница 14 из 38 4-2 Stage 1 @ Placing the Specimen (Figs. 6 & 7) Put the specimen in the center of the stage. When Using a 35 mm Petri Dish }With the CKX41, a 35 mm petri dish can be mounted directly on the stage provided that the standard stage center plate is in use. 1. With the CKX31, put the provided 35 mm
Страница 15 из 38 CKX41/CKX31 4-3 Observation Tube 1 Adjusting the Interpupillary Distance (Figs. 8 to 10) When adjusting the interpupillary distance, take care not to let your fingers get caught in the gaps of the binocular section. With the CKX31 While looking through the eyepieces, move both eyepieces until the
Страница 16 из 38 @ 2 Adjusting the Diopter (Figs. 11 to 13) With the CKX31 1. While looking through the left eyepiece with your left eye, turn the coarse and fine focus adjustment knobs to bring the specimen into focus. 2. While looking through the right eyepiece with your right eye, turn only the diopter
Страница 17 из 38 CKX41/CKX31 4 1 2 WHN10X-H Fig. 15-1 WHB10X-H 4 3 5 Fig. 15-2 Using the Eyepiece Micrometer Disk (Figs. 15) }When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece micrometer disk can be inserted in one of them. When the eyepiece does not have a diopter adjustment mechanism, however, it is
Страница 18 из 38 5 Selecting the Light Path (U-TR30-2 Only) (Fig. 16) Slide the light path selector knob 1 to select the desired light path. Light Path Selector Knob @ Symbol Intensity Ratio Applications Pushed in 100% for binocular eyepieces Observation of dark specimens Middle position 20% for binocular
Страница 19 из 38 CKX41/CKX31 4-4 Illumination Column 1 Using the Filters }Using appropriate filters according to the purposes allows you to observe and photograph specimens more effectively. Particularly, the use of the LBD filter is recommended in observation and photomicrography because it renders more neutral
Страница 20 из 38 4-5 Objective Correction Collars 1 @ ² Fig. 20 16 Correction Collar (Fig. 20) }A culture microscope is designed to observe specimens contained in vessels of various bottom thickness values. In order to achieve optimum objective performance of the culture microscope, the LUCPlanFLN20X, 40X, 60X,
Страница 21 из 38 CKX41/CKX31 PHASE CONTRAST OBSERVATION The following two slider units are available for phase contrast observation. Mount a slider onto the microscope and replace the objectives with phase contrast compatible objectives. Model Name Description Applicable Objectives (Note) Ph precentering slider
Страница 22 из 38 @ ³ 2 ² Fig. 21 3 @ ² Fig. 22 18 (Fig. 21) }When the IX2-SL phase contrast slider is used, it is required to attach an optical element matching the phase objective in use. 1. Hold the IX2-SLPH2 optical element 1 so that engraving faces upward and drop in the optical element in the empty position 2.
Страница 23 из 38 CKX41/CKX31 @ ² Fig. 23 ³ | Fig. 24 4 Centering the Light Annulus (Figs. 23 & 24) # The IX2-SLP does not need to be centered. However, as the phase contrast effect near the vessel edge tends to drop because the light annulus image may be deformed due to the liquid surface curvature (surface
Страница 24 из 38 PHOTOMICROGRAPHY & TV OBSERVATION C-mount TV camera Photomicrographic system PM10 PM20 PM30, etc. (Note) The system becomes unstable when a large camera back is used. TV camera TV camera mount adapter U-BMAD U-IMAD U-CMAD3, etc. TV adapter U-PMTVC PE photo eyepiece Straight photo tube U-SPT TV
Страница 25 из 38 CKX41/CKX31 TROUBLESHOOTING GUIDE Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please
Страница 26 из 38 Trouble f ) One side of image is blurred. Cause Remedy Page The revolving nosepiece is not correctly Make sure that the revolving nosepiece engaged. clicks properly into place. 8 The specimen is not correctly mounted Place it correctly on the stage. on the stage. 10 The optical performances
Страница 27 из 38 CKX41/CKX31 SPECIFICATIONS Specification Item CKX31 CKX41 1. Optical system UIS2/UIS (Universal Infinity System) optical system 2. Illumination Built-in transmitted Koehler illumination High-intensity halogen bulb 6V30WHAL (PHILIPS 5761) (Average life time: Approx. 100 hr. when used as directed)
Страница 28 из 38 Specification Item CKX31 7. UIS2 Objectives (UIS series objectives can also be used.) 24 Brightfield PlanCN4X PlanCN10X PlanCN20X PlanCN40X PlanCN60X PlanCN100XO LUCPlanFLN20X LUCPlanFLN40X LUCPlanFLN60X N.A. 0.10, N.A. 0.25, N.A. 0.40, N.A. 0.65, N.A. 0.80, N.A. 1.25, N.A. 0.45, N.A. 0.60, N.A.
Страница 29 из 38 CKX41/CKX31 ASSEMBLY 9-1 Assembly Diagram The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assembly. # When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts or touching glass
Страница 30 из 38 9-2 Detailed Assembly Procedures 1 @ (Fig. 25) }Use only the designated high-intensity halogen bulb 6V30WHAL (PHILIPS 5761). To prevent reduced bulb life or cracking, do not touch the bulb with bare hands. If fingerprints are accidentally left on the bulb, wipe the bulb with a soft cloth. · Holding
Страница 31 из 38 CKX41/CKX31 4 Mounting the Objectives (Figs. 28 & 29) # First raise the revolving nosepiece slightly to remove the transportation pad on the nosepiece’s base. }Keep the transportation pad in a safe place. You will need it when the equipment is sent for repair or transported to another location. 1.
Страница 32 из 38 6 ² Mounting the Stage Center Plate (CKX41 Only) (Fig. 31) Fit the standard stage center plate 1 into the opening on the stage. }Turn the center plate so that the notch 2 faces to the front for easy confirmation of the objective tip. # When using the glass stage center plate, set it in the
Страница 33 из 38 CKX41/CKX31 9 ² Mounting the Filters (Figs. 34 & 35) Let the filters cool down sufficiently before replacing them. Take out the filter holder 1 and insert the required filters 2. # Push the filter down to the bottom as shown in Fig. 35 so that it does not tilt. If the filter is inclined or is not
Страница 34 из 38 PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no
Страница 35 из 38 CKX41/CKX31 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Approval Organization Printed or Embossed Harmoniza- Alternative Marking Utilizing tion Marking (May be located on Black-Red-Yellow Thread (Length jacket or insulation of internal wir- of color
Страница 37 из 38 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan EC REP Wendenstraße 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. One Corporate Drive, Orangeburg, NY 10962, U.S.A. 491B River Valley Road, #12-01/04 Valley Point