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USER INSTRUCTIONS

MITICO

(

-2L

)

LAS-BOB

LAS-MC

MOTORIZED INTEGRATED TIRF

ILLUMINATION COMBINER

LASER SAFETY BREAK-OUT BOX

LASER MANUAL CONTROL

This instruction manual describes the Olympus

MITICO illumination combiner with

accessories. To ensure safety, obtain optimum performance and familiarize yourself fully with the
use of this product, we recommend that you study this manual thoroughly before operation.
Together with this manual, please also read the imaging system manuals, the laser manual and
the instruction manual of the microscope in order to understand overall operation methods.
Retain this manual in an easily accessible place near a system for future reference.

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Оглавление инструкции

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® USER INSTRUCTIONS MITICO(-2L) LAS-BOB LAS-MC MOTORIZED INTEGRATED TIRF ILLUMINATION COMBINER LASER SAFETY BREAK-OUT BOX LASER MANUAL CONTROL This instruction manual describes the Olympus MITICO illumination combiner with accessories. To ensure safety, obtain optimum performance and familiarize
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OLYMPUS SOFT IMAGING SOLUTIONS GMBH Johann-Krane-Weg 39 D-48149 Münster Tel: +49 89 - 89 55 805 660 Fax +49 89 - 89 55 805 6606 Email: info@olympus-sis.com www.olympus-sis.com
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Imaging Excellence We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever
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Illumination Combiner, User Manual Contents Contents 1 1.1 1.2 1.3 Introduction .................................................................................................1 Abstract ........................................................................................................2
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Illumination Combiner, User Manual Chapter 1 –Introduction 1 Introduction Thank you very much for purchasing Olympus Soft Imaging Solutions' state of art motorized illuminator system, and for your confidence in our products and service. It is Olympus Soft Imaging Solutions' main objective to
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2 Chapter 1 – Introduction Motorized multi-port illumination combiner MITICO with fiber port for widefield illumination 1.1 Abstract In the following sections you will find a detailed description of the components of the illuminator system, handling and alignment instructions and how to maintain
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Illumination Combiner, User Manual Chapter 1 –Introduction 1:2003, Sect. 4.4 and 10.2). Furthermore, every laser unit must have a key-activated main power switch in the sense of EN 60825-1:2003, Sect. 4.5). • Provided that the laser units can be operated independently they must have their own
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4 Chapter 1 – Introduction i – Either a TIR-LHA adaptor to couple a standard fluorescence lamp housing – or a TIRMT-CON to couple a MT10 / MT20 illumination system j A widefield illumination system – Either a standard fluorescence lamp housing, e.g., U-LH100HGAPO – or a MT10 / MT20 illumination
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Illumination Combiner, User Manual Chapter 2 – Safety Symbols 2 Safety Symbols The deactivation of the safety systems or the dismantling of different components by the operator is required in certain operational modes and in case of illumination alignment. As a result the Olympus motorized
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6 Chapter 2 – Safety Symbols The following symbols are placed on the Olympus illuminator system. Study the meaning of the symbols and always use the equipment in the safest possible manner. General symbols Symbol Explanation Indicates the use of a laser beam. Take special care in handling that
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Chapter 2 – Safety Symbols Illumination Combiner, User Manual Labels for the microscope components are included in the shipping list of the illuminator system. Please paste them on the microscope upon system installation. If any label is missing please ask your Olympus representative for additional
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8 Chapter 2 – Safety Symbols
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Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions 3 Safety Precautions and Handling Instructions The Olympus illuminator system must be used with laser products belonging to the Laser Safety Class 3B. Class 3B lasers are medium power lasers with an output
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10 Chapter 3 – Safety Precautions and Handling Instructions 3.1 Laser Safety Precautions Caution: Control or adjustments using procedures other than those prescribed in this manual will lead to hazardous laser beam exposure. To avoid hazardous situations strictly follow the laser safety
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Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions Direct exposure to the laser light is possible at the following positions: • The laser system’s housing at the fiber port if the fiber is removed • The exit of the laser fiber • The fiber coupling of the
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12 Chapter 3 – Safety Precautions and Handling Instructions
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Illumination Combiner, User Manual 4 Chapter 4 – MITICO and Peripherals Overview MITICO and Peripherals Overview The following chapter describes all components and interfaces of the Olympus tem including all laser safety devices. 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 4.10 4.11 4.12 4.13 4.14
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14 Chapter 4 – MITICO and Peripherals Overview 4.1 Setup Scheme To MT20 illumination system To real-time controller or USB adaptor Laser fibers Laser safety shutter inside TIRMT-CON MITICO Laser safety stage cover LASBOB Laser safety ocular shutter IX81, top view Scheme of an IX81 microscope with a
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Illumination Combiner, User Manual Chapter 4 – MITICO and Peripherals Overview 4.2 The Laser Safety Features The laser safety concept of the illuminator system consists of the following modules: — A double-redundant laser safety shutter in front of the exit optics of the combiner — A laser safety
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16 Chapter 4 – MITICO and Peripherals Overview Laser safety stage cover frame Laser safety stage cover • The 5-pin ocular shutter cable must be connected to the stage cover and this additionally to the 5pin STAGE COVER plug of LAS-BOB laser safety breakout box. • One of the two SAFETY SHUTTER plugs
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Illumination Combiner, User Manual Chapter 4 – MITICO and Peripherals Overview 4.3 The MITICO Features – an Overview The MITICO motorized integrated TIRF illumination combiner features — Four FCP8/FCP laser fiber ports with four-axis alignment — Two Lemo plugs to connect it via ODB communication
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18 Chapter 4 – MITICO and Peripherals Overview 4.4 The LAS-BOB Laser Safety Break-out Box It features on the front panel — Four INTERLOCK 4 – 3 – 2 – 1 cinch plugs to connect it with the INTERLOCK connectors of Olympus Soft Imaging Solutions laser systems (Other lasers may need adaptors.) — Four
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Illumination Combiner, User Manual MITICO and Peripherals Overview Chapter 4 – A BINOCULAR button to bridge the laser safety ocular shutter in maintenance mode so that the specimen can be observed via ocular with laser illumination. This is not a toggle button; it needs to be kept pressed in order
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20 Chapter 4 – MITICO and Peripherals Overview 4.7 The Remote Interlock Connector of the Olympus Soft Imaging Solutions ODB Laser Systems An INTERLOCK connector can be found at the front panel of all Olympus Soft Imaging Solutions laser systems as Class 3B laser products. This remote interlock
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Illumination Combiner, User Manual Chapter 4 – MITICO and Peripherals Overview The slider has to be moved all the way to the right to operate either with the TIRFM laser only or to combine TIRFM illumination with widefield illumination. The polarizing beam splitter cube blocks 50% of the widefield
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22 Chapter 4 – MITICO and Peripherals Overview 4.10 Laser Clean-up Filter Sliders Some lasers may require clean-up filters to suppress background light. Standard 25 mm clean-up filters can be inserted into the respective slider of each laser line in the MITICO illumination combiner. The sliders can
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Illumination Combiner, User Manual Chapter 4 – MITICO and Peripherals Overview 2. Set the laser lines to widefield position via the TIRF control window; see Chapter 6. 3. Open the laser shutter and observe the beam as it transmits the translucent insert in the laser safety stage cover. 4. Unlock
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24 Chapter 4 – MITICO and Peripherals Overview Be sure to wear the adequate eye protection for the laser wavelength and laser power in use. Once the MITICO illumination combiner is aligned, be sure to switch back to User Mode to activate the safety devices. According to the regulations trained
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Illumination Combiner, User Manual Chapter 5 – Modes of Operation 5 Modes of Operation There are two modes of operation, the user mode and the maintenance mode. In user mode no laser radiation can exit the system. The maintenance mode is for setting-up and adjusting the system. Only trained
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26 Chapter 5 – Modes of Operation 5.1 The User Mode All hardware has to be connected properly to operate the system in User mode. The user mode ensures that the user can operate the system without being exposed to the laser beam. It is possible to have full control of the various devices via
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Chapter 5 – Modes of Operation Illumination Combiner, User Manual 6. Open the high-speed laser shutter either via software (with the REMOTE switch of the LASMC manual control set to ( I ) in case TIRF Control.exe is used) or by switching the corresponding LASER 1, 2, 3, 4 switch of the LAS-MC
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28 Chapter 5 – Modes of Operation 5.2.1 Exchanging Microscope or Camera Hardware Before exchanging hardware, always consider the compliance of the new hardware to the requirements of TIRF microscopy and the laser equipment. Ask your Olympus specialist for advice in case of uncertainty. The best way
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Illumination Combiner, User Manual Chapter 5 – Modes of Operation 5.2.4 Observing the Specimen under TIRF Illumination Through the Ocular Authorized and trained personnel can observe the specimen in Maintenance mode via the ocular when taking the safety precautions into account. Before observation
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30 Chapter 5 – Modes of Operation
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Illumination Combiner, User Manual Chapter 6 – Using the MITICO Illumination Combiner 6 Using the MITICO Illumination Combiner The illuminator system and the Olympus Soft Imaging Solutions laser systems are fully integrated in the cell^M / cell^R imaging software. In case the illuminator system is
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32 Chapter 6 – Using the MITICO Illumination Combiner 6.1 The cell^TIRF Control Software cell^TIRF.exe icon Execute the cell^TIRF.exe file to start the software. The first step is to click the Connect button to establish the communication between PC and the MITICO illumination combiner. See below
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Illumination Combiner, User Manual Chapter 6 – Using the MITICO Illumination Combiner example, in a typical four laser system a 405 nm laser must be connected to the top port, a 640 nm laser to the lowest port and a 488 nm and a 561 nm laser correspondingly to the second and third ports from top.
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34 Chapter 6 – Using the MITICO Illumination Combiner Show: The following check boxes activate/deactivate the display of features of the cell^TIRF Control window. The graph It shows a plot of penetration depth vs. incident angle for each wavelength in the respective color. Graph. Check/uncheck this
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Illumination Combiner, User Manual Chapter 6 – Using the MITICO Illumination Combiner Max. Angle [°]. This is the calculated maximum angle for the active wavelength that can be obtained with the objective in use in dependency of the current settings. This corresponds to the position where the beam
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36 Chapter 6 – Using the MITICO Illumination Combiner Illumination Parameters Show Illumination Parameters. Check/uncheck this box to display/hide additional, calculated, optical parameters. Incident Angle [°]. This is the angle of the laser beam relative to the normal of the glass-to-medium
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Illumination Combiner, User Manual Chapter 6 – Using the MITICO Illumination Combiner ODB Lasers. This field lists all Olympus Soft Imaging Solutions lasers connected via the ODB device bus. You can use the Illumination Parameters to check if your assumption of the refractive index of the medium is
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38 Chapter 6 – Using the MITICO Illumination Combiner 6.2 MITICO Control via the cell^R Software 6.2.1 System Configuration 1. Start the ObsConfig software to configure the excitation filters 2. Make sure that the lasers and corresponding image types are configured on pages Microscope Laser and
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Illumination Combiner, User Manual 6.2.2 Operating Chapter 6 – Using the MITICO Illumination Combiner MITICO Optical Parameters These parameters serve to calculate the theoretical values of critical angle, penetration depth and so on. Objective. Select the objective in use from the shortlist. It
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40 Chapter 6 – Using the MITICO Illumination Combiner 0 and about 2.8. The maximum value will be set automatically if the number typed is higher than can be reached by the system. Penetration Depth [nm]. These are the value calculated from the current settings. See below for the definition of PD.
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Illumination Combiner, User Manual Chapter 6 – Using the MITICO Illumination Combiner changing the Refract. Ind. Sample value. If you prefer, however, not to change the refractive index of the sample you can fine adjust the system in the following way: 1. Click the Critical Angle button to move the
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42 Chapter 6 – Using the MITICO Illumination Combiner It is, however, possible to control the beam position during the experiment in order to change penetration depth or switch from TIRF to widefield illumination, for example. The corresponding TIRF command icon is placed on the Lightsource
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Illumination Combiner, User Manual Chapter 6 – Using the MITICO Illumination Combiner 43
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44 Chapter 6 – Using the MITICO Illumination Combiner
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Illumination Combiner, User Manual Chapter 7 – Specifications and Technical Data 7 Specifications and Technical Data 45
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46 Chapter 7 – Specifications and Technical Data Specifications and Technical Data Dimensions and Weight (MITICO): H 182 x W 420 x D 282 mm (incl. exit optics tube and flange: 154 mm); 7 kg Power supply rating: 24 V, 1.88 A, DC power supply connected to LAS-BOB Enclosure rating: IP20 Operating
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® OLYMPUS EUROPA HOLDING GMBH Postfach 10 49 08, 20034, Hamburg, Germany OLYMPUS AMERICA INC. 3500 Corporate Parkway, Center Valley, PA 18034, USA OLYMPUS AUSTRALIA PTY. LTD. 31 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia OLYMPUS LATIN AMERICA, INC. 6100 Blue Lagoon Drive, Suite 390
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