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Illumination Combiner, User Manual
Chapter 4 –
MITICO and Peripherals Overview
17
4.3
The MITICO Features – an Overview
The MITICO motorized integrated TIRF illumination combiner features
—
Four FCP8/FCP laser fiber ports with four-axis alignment
—
Two Lemo plugs to connect it via ODB communication cables with
a) – either the real-time controller inside the PC of a cell^M / cell^R imaging system
– or the AD-USB-ODB bus adaptor box and
b) other OSIS ODB devices (in daisy chain)
—
A 5-pin plug to connect with the SAFETY SHUTTER plug of the LAS-BOB laser safety break-
out box
—
Two red safety shutter status diodes
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A green ODB communication status diode
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A green power status diode
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An iris diaphragm field stop for each laser line
—
A laser clean-up filter slider for each laser line
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A laser beam focus slider for each laser line
—
A switch to insert a FRAP focusing lens into the beam path of line 1
—
A light path selector slider to switch from 100%TIRF with 50% widefield illumination to 100%
widefield illumination
—
A widefield fluorescence illumination port to mount
a) either a TIRMT-CON widefield fiber illumination coupling if a fiber-coupled fluorescence illu-
mination systems MT10 or MT20 is to be used or
b) a TIR-LHA adaptor if a standard fluorescence lamp housing is to be used
—
An epi-fluorescence illuminator tube to connect it with the microscope frame. This contains a
spacer unit with tube, which has to be removed for ZDC-equipped microscopes.
Spacer for standard
microscope frames
Spacer removed
for ZDC frames