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Chapter 4 –
MITICO and Peripherals Overview
4.10
Laser Clean-up Filter Sliders
Some lasers may require clean-up filters to suppress background light. Standard 25 mm clean-up fil-
ters can be inserted into the respective slider of each laser line in the MITICO illumination combiner.
The sliders can be removed and inserted easily.
4.11
Focusing the Laser Lines
In order to obtain the best possible TIRF performance it is necessary to focus each laser beam into the
back focal plane of the objective. This ensures that a narrow, collimated beam exits the objective. This
is the prerequisite for a pronounced transition from widefield to TIRF illumination and a good Z resolu-
tion.
In order to check the focusing, do the following:
1.
Move the TIRF objective and a suitable mirror cube into position without any sample
mounted and the laser safety stage cover in place.
Removable sliders
to insert clean-up
filters for each
laser line
Focus sliders
for each laser
line