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10
Chapter 3 –
Safety Precautions and Handling
Instructions
3.1
Laser Safety Precautions
Caution: Control or adjustments using procedures other than those prescribed in this
manual will lead to hazardous laser beam exposure.
To avoid hazardous situations strictly follow the laser safety instructions below.
1.
Before operating an Olympus
scanning FRAP system check if all devices are
attached correctly and all apertures are closed.
2.
The usage of a Class 3B laser is only permitted under the control of a
laser safety officer
.
3.
A Class 3B laser
can cause irreversible damage to your eyes
. Thus do not stare into the
laser beam or illuminated fiber. Make sure to wear adequate eye protection during operation
of the laser in maintenance mode.
4.
Always operate the
scanning FRAP system in
user
mode unless you underwent a
detailed training in operating the system in
maintenance
mode. Inform your laser safety
officer if you intend to operate the system in
maintenance
mode. The
user
mode is the
safest manner to operate the system. Opening the stage cover or the binocular slider will
interrupt the laser beam in this mode.
5.
Only trained Olympus service personnel is allowed to perform the installation and alignment
procedures described in the
Service Manual.
6.
A person in charge with the laser alignment or performing scientific experiments while
operating the laser in
maintenance
mode must be aware of the safety precautions adequate
to the TIRF microscope setup. He/she is responsible for the safety of any person present in
the room while operating the lasers in
maintenance
mode.
7.
The focused laser beam or any UV light emitted from the microscope nosepiece
may injure
the skin
through the objective exit lens and through a free nosepiece position. Thus, avoid
exposing the hands to the laser beam when working on the microscope.
8.
Do not bend, step on or pull excessively on the laser fibers. This could damage a fiber and
cause hazardous beam leakage. In case of a damaged fiber, switch off the laser immediately
and contact Olympus.
9.
Be sure all apertures of your microscope system stay closed or are connected while
operating the laser. A change in camera or any microscope part should be performed only
when the laser is turned off.
10.
Never cover the air outlet of the laser to prevent overheating.