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Manual
Contents
Contents
1 Introduction.........................................................................................................1
1.1
Abstract ........................................................................................................2
1.2
General .........................................................................................................3
1.3
Components .................................................................................................3
2 Safety Symbols ...................................................................................................5
3 Safety Precautions and Handling Instructions ...............................................9
3.1
Laser Safety Precautions ............................................................................10
3.2
General Safety Precautions ........................................................................11
4
Overview and Laser Safety Setup ...................................................13
4.1
Setup Scheme ............................................................................................14
4.2
The Laser Safety Features – Overview and Check .....................................15
4.3
Mounting the Laser Safety Kit ....................................................................17
4.4
Connecting the
Control Unit .......................................................18
4.5
Connecting the LAS-BOB Laser Safety Break-out Box .............................19
4.6
The Laser Shutter Manual Control..............................................................20
4.7
Key Control .................................................................................................21
4.8
The Remote Interlock Connector of the Olympus Soft Imaging Solutions
ODB Laser Systems....................................................................................22
5 Modes of Operation..........................................................................................23
5.1
The User Mode ...........................................................................................24
5.2
The Maintenance Mode ..............................................................................25
5.2.1
Exchanging Microscope or Camera Hardware...........................................25
5.2.2
Access to the Specimen without Deactivating the FRAP System..............26
5.2.3
Observing the Specimen Through the Ocular ............................................26
6 Operating the
System.......................................................................29
6.1
System Configuration .................................................................................30
6.2
The FRAP GUI in
...............................................................31
6.3
in the Experiment Manager ........................................................33
6.3.1
FRAP Experiments......................................................................................33
6.3.2
Fire On Click ...............................................................................................36
6.3.3
Pattern Bleaching – Fluorescence Speckle Microscopy ............................37
7 Appendix ..........................................................................................................39
7.1
Laser Safety Stage Cover Dimensions .......................................................40