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Manual
Chapter 7
– Specifications, Technical data
41
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Страница 1 из 52 ® I INSTRUCTIONS SOFTWARE MODULE AND LASER SAFETY FOR THE HIGH-SPEED SCANNING FRAP SYSTEM This instruction manual describes the configuration and usage of the scanning FRAP Imaging Stations. To ensure safety, obtain optimum system within the Olympus performance and familiarize yourself fully with
Страница 3 из 52 III Imaging Excellence We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever
Страница 7 из 52 Manual Chapter 1 –Introduction 1 Introduction Thank you very much for purchasing Olympus Soft Imaging Solutions' state of art scanning FRAP system and for your confidence in our products and service. It is Olympus Soft Imaging Solutions' main objective to provide you with solutions able to meet
Страница 8 из 52 2 Chapter 1 – Introduction scan head mounted on an IX81 1.1 Abstract In the following sections you will find a detailed description of the components of scanning FRAP system, handling and alignment instructions and how to maintain the performance of your FRAP microscope. Keep in mind that all
Страница 9 из 52 Manual Chapter 1 –Introduction 1.2 General The MITICO motorized integrated TIRF illumination combiner is compatible with the Olympus microscope frames IX81S1F-FRAP, IX81S1F-FRAP-ZDC, and IX71S1F-FRAP, as well as a range of diode laser systems between 405 nm and 700 nm provided that they meet the
Страница 10 из 52 4 Chapter 1 – Introduction a illuminator featuring two fiber ports, each with adjustable line focus b control unit
Страница 11 из 52 Manual Chapter 2 – Safety Symbols 2 Safety Symbols The deactivation of the safety systems or the dismantling of different components by the operator is required in certain operational modes and in case of illumination alignment. As a result the Olympus scanning FRAP system is to be classified in
Страница 12 из 52 6 Chapter 2 – Safety Symbols The following symbols are placed on the Olympus scanning FRAP system. Study the meaning of the symbols and always use the equipment in the safest possible manner. General symbols Symbol Explanation Indicates the use of a laser beam. Take special care in handling that
Страница 13 из 52 Chapter 2 – Safety Symbols Manual 1: scan head 2: microscope frame, right side 3: fluorescence turret and C-mount 4: laser safety ocular shutter 5: laser safety stage cover 7
Страница 15 из 52 Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions 3 Safety Precautions and Handling Instructions The Olympus scanning FRAP system must be used with laser products belonging to the Laser Safety Class 3B. Class 3B lasers are medium power lasers with an output
Страница 16 из 52 10 Chapter 3 – Safety Precautions and Handling Instructions 3.1 Laser Safety Precautions Caution: Control or adjustments using procedures other than those prescribed in this manual will lead to hazardous laser beam exposure. To avoid hazardous situations strictly follow the laser safety
Страница 17 из 52 Illumination Combiner, User Manual Chapter 3 – Safety Precautions and Handling Instructions Direct exposure to the laser light is possible at the following positions: • The laser system’s housing at the fiber port if the fiber is removed • The exit of the laser fiber • The fiber coupling of the
Страница 18 из 52 12 Chapter 3 – Safety Precautions and Handling Instructions
Страница 19 из 52 Manual 4 Chapter 4 – Overview and Laser Safety Setup Overview and Laser Safety Setup The following chapter describes all components and interfaces of the Olympus system including all laser safety devices and how they are installed and connected. 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 scanning FRAP Setup
Страница 20 из 52 14 Overview and Laser Safety Setup Chapter 4 – 4.1 Setup Scheme Laser fibers LAS-BOB scan head Laser safety stage cover FRAP lasers with safety shutters Laser safety ocular shutter LAS-MC IX81, top view controller Scheme of an IX81 microscope with a PC scanning FRAP system, two lasers and the laser
Страница 21 из 52 Manual Chapter 4 – Overview and Laser Safety Setup 4.2 The Laser Safety Features – Overview and Check The laser safety concept of the scanning FRAP system consists of the following modules: – A laser safety ocular shutter – A laser safety stage cover – An emergency interrupter located on the LAS-MC
Страница 22 из 52 16 Chapter 4 – Overview and Laser Safety Setup Laser safety stage cover Laser safety stage cover • The 5-pin ocular shutter cable must be connected to the stage cover and this additionally to one of the 5-pin SAFETY INTERLOCK plugs at the back panel of the control unit. • The other 5-pin SAFETY
Страница 23 из 52 Manual Chapter 4 – Overview and Laser Safety Setup 4.3 Mounting the Laser Safety Kit 1. Remove the ocular from the microscope frame and mount the ocular laser safety shutter onto the ocular tube (with the cable on the right hand side) and fix it as shown in the photo below. 2. Mount the ocular back
Страница 24 из 52 18 Chapter 4 – Overview and Laser Safety Setup 6. Connect the second plug of the laser safety stage cover with the 5-pin female STAGE COVER plug of the scanning illuminator using the corresponding cable. Translucent insert 4.4 Connecting the 5-pin plugs Control Unit The installation of the scanning
Страница 25 из 52 Manual Chapter 4 – 3. Connect the PC RS232 plug at the back panel of the the system PC. Overview and Laser Safety Setup control unit with a serial port of 4. Connect the cable coming from the scan head with the multi-pin plug at the front panel of the control unit. 5. Connect the TRIGGER IN TTL BNC
Страница 26 из 52 20 Chapter 4 – Overview and Laser Safety Setup 2. Connect the 5-pin female STAGE COVER plug at the LAS-BOB back panel with one of the control unit using 5-pin female SAFETY INTERLOCK plugs at the back panel of the the corresponding cable. Make sure the control unit is connected with the laser
Страница 27 из 52 Manual Chapter 4 – Overview and Laser Safety Setup – A mode lock with key to allow authorized and trained personnel to switch from standard USER MODE to MAINTENANCE (see Chapter 5, Modes of Operation). – A red emergency interrupter that closes all safety shutters when pressed. – A BINOCULAR button
Страница 28 из 52 22 Chapter 4 – Overview and Laser Safety Setup 4.8 The Remote Interlock Connector of the Olympus Soft Imaging Solutions ODB Laser Systems An INTERLOCK connector can be found at the front panel of all Olympus Soft Imaging Solutions laser systems as Class 3B laser products. This remote interlock
Страница 29 из 52 Manual Chapter 5 – Modes of Operation 5 Modes of Operation There are two modes of operation, the user mode and the maintenance mode. In user mode no laser radiation can exit the system. The maintenance mode is for setting-up and adjusting the scanning FRAP system. Only trained personnel are allowed
Страница 30 из 52 24 Chapter 5 – Modes of Operation 5.1 The User Mode All hardware has to be connected properly to operate the system in User mode. The user mode ensures that the user can operate the system without being exposed to the laser beam. It is possible to have full control of the various devices via
Страница 31 из 52 Manual Chapter 5 – Modes of Operation To protect your specimen from photo-bleaching, close the laser shutter (by reversing step 5) whenever suitable, even if you interrupt observation only for a short time. 5.2 The Maintenance Mode Actions in maintenance mode Permitted Action Remarks Exchanging
Страница 32 из 52 26 Chapter 5 – Modes of Operation 5.2.2 Access to the Specimen without Deactivating the FRAP System For some applications it is necessary to manipulate the specimen during an experiment. In User mode, however, the laser safety stage cover has to be in place; otherwise the FRAP system is deactivated
Страница 33 из 52 Manual Chapter 5 – Modes of Operation Laser safety ocular shutter, slider OUT 3. Hold a sheet of paper in front of the eyepieces. 4. Open the laser safety shutters of the lasers by pressing the red ocular safety interlock bridge button of the LAS-MC manual control. Check the sheet of paper to
Страница 35 из 52 Manual Chapter 6 – Operating the 6 Operating the System System The scanning FRAP system and the Olympus Soft Imaging Solutions laser systems are fully integrated in the imaging software This chapter describes the configuration and usage of the scanning FRAP system. 6.1 6.2 6.3 6.3.1 6.3.2 6.3.3
Страница 36 из 52 30 Chapter 6 – Operating the System 6.1 System Configuration 1. Start the ObsConfig software to configure the excitation filters 2. Make sure that the FRAP lasers are configured on pages Microscope Laser as described in the laser manual. 3. Go to page Microscope FRAP. In case this page is not
Страница 37 из 52 Manual Chapter 6 – Operating the System 8. TTL Synchronization, FRAP Trigger OUT -> DIG I/O. Select from the short list the TTL IN imaging PC front panel that is connected with the TRIGGER connection at the OUT of the control unit. 9. Exit the software with OK. 6.2 The FRAP GUI in This interface is
Страница 38 из 52 32 Chapter 6 – Operating the System The calibration is valid only for the objective in place during the calibration. A later manual change of the objective does not cause a change of the status display. However, a recalibration is required. Set spot size. Click here to open the Enter spotsize
Страница 39 из 52 Manual Chapter 6 – Operating the System because the software will detect the bright spot when it is reasonably close. Increase the step size by keeping the Ctrl key pressed while using the arrow key. Keep the Shift key pressed to reduce it. Once the spot is at the position of the red circle click
Страница 40 из 52 34 Chapter 6 – Operating the System after bleaching. The Acquire radio buttons and the Condition parameters are deactivated. Manual start during experiment is not possible. FLIP (Fluorescence Loss In Photobleaching). In this experiment the ROIs are bleached in Every Cycle and acquisition always
Страница 41 из 52 Manual Chapter 6 – Operating the System Manual start during experiment. This check box is active only if the Advanced experiment is selected. If the option is checked the command icon shows a hand symbol. The command icon turns into a button. FRAP command icon with Manual start symbol What will
Страница 42 из 52 36 Chapter 6 – Operating the System Edge accuracy. The FRAP scanner controller composes the ROIs to be scanned out of subrectangles. The smaller the sub-rectangles are the higher is the resulting correspondence with the ROIs. Set the accuracy by use of the slider or by typing a value. The default
Страница 43 из 52 Manual Chapter 6 – Operating the System is the first one within each cycle. At one point in time during a certain cycle a Click is being applied. The Fire will only be carried out as soon as the next cycle is being started. Thus, while the manual Click is unsynchronized, of course, the Fire
Страница 45 из 52 Manual Chapter 7 – Specifications, Technical data 7 Appendix This chapter lists specifications and technical data of the 7.1 system. Laser Safety Stage Cover Dimensions .......................................................40 39
Страница 46 из 52 40 Chapter 7 –Specifications, Technical Data 7.1 Laser Safety Stage Cover Dimensions The laser safety stage cover must be secured to the microscope stage with the M4x5 screws provided. If the microscope stage does not have the necessary pre-drilled holes, this simple modification must be performed
Страница 47 из 52 Manual Chapter 7 – Specifications, Technical data 41