Acquiring specific images

33 

The camera is aligned parallel to the XY-stage. The angle between camera and 
stage should be smaller than 1°. 

1.  Switch to the "Acquisition" layout. To do this, use, e.g., the 

View > Layout 

> Acquisition

 command. 

2. In 

the 

labSens

 tool window, click the button with the objective that you 

want to use for the acquisition of the stitched image. 

3.  Switch to the live mode, and select the optimal settings for your 

acquisition in the 

Camera Control

 tool window. Pay special attention to 

setting the correct exposure time. This exposure time will be used for all 
of the stitched image's individual images. 

4.  Find the position on the sample at which you want to start acquiring the 

stitched image. 

5.  Finish the live mode. 

6. Activate 

the 

Process Manager

 tool window. To do this, use, e.g., the 

View > Tool Windows > Process Manager

 command. 

7. Select 

the 

Manual Processes

 option. 

8. Click 

the 

Manual MIA 

 button.  



The button will appear clicked. You can recognize this status by the 
button's colored background.  



 The 

Manual MIA

 group will be automatically displayed in the tool 

window. 

9.  Make quite certain that the 

Auto Align

 button appears clicked. It should 

then look like this: 

. 



Then your software will search for the same image structures in 
neighboring individual images. The stitched image will be put 
together in such a way that image areas that are the same will be 
superimposed. 



A tip if your system is equipped with a motorized XY-stage: Some 
motorized microscope stages work so precisely that you can 
dispense with an overlapping of the individual images. If you want to 
acquire the individual images edge to edge, release the 

Auto align

button. The individual images will now be set next to one another just 
as they were acquired.  

10. Click the 

Start 

 button. 



Your software switches into the live mode. 

11.  Bring the sample into focus.  

12.  Click on one of the arrow buttons to set the side of the current image at 

which the next image is to be arranged. For example, click this button 

 if the next image is to be laid to the right of the current image. 



Your system now acquires an image at the current position on the 
sample. In the image window, you now see on the left (1) the 

Prerequisite  

Selecting the 

objective 

Setting the image 

quality 

Selecting the 

acquisition process 

Selecting the 

acquisition 

parameters 

Acquiring a 

stitched image